Integrating proteomics with electrochemistry for identifying kinase biomarkers.

Integrating proteomics with electrochemistry for identifying kinase biomarkers† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c5sc00560d Click here for additional data file. Click here for additional data file.

Levels of C-reactive protein, creatine kinase-muscle and aldolase A are suitable biomarkers to detect the risk factors for osteoarthritic disorders: A novel diagnostic protocol

Background: C-reactive protein (CRP), creatine kinase-muscle (CK-MM) and aldolase A (AldoA) levels are predicted to be realistic biomarkers of osteoarthritic disorders (OADs). The objective of the study was to evaluate the levels of CRP, CK-MM, and AldoA and determine their correlations with risk factors such as inflammation, muscle degeneration, and skeletal muscle damage for OADs. Methods: B...

Electrochemistry-based approaches to low cost, high sensitivity, automated, multiplexed protein immunoassays for cancer diagnostics.

Early detection and reliable diagnostics are keys to effectively design cancer therapies with better prognoses. The simultaneous detection of panels of biomarker proteins holds great promise as a general tool for reliable cancer diagnostics. A major challenge in designing such a panel is to decide upon a coherent group of biomarkers which have higher specificity for a given type of cancer. The ...

Identifying Low-Abundance Biomarkers

B iomarkers are clinical, molecular, or image-based measurable parameters that can characterize an individual’s specific biological state, whether normal, pathological, or in response to treatment. A biomarker is considered of clinically valuable if (1) it can be measured repeatedly with accuracy and relatively rapid clinical turnaround, (2) it provides unique, superior information on patient s...

Proteomics analysis of serum protein profiling in pancreatic cancer patients by DIGE: up-regulation of mannose-binding lectin

Background Pancreatic cancer has significant morbidity and mortality worldwide. Good prognosis relies on an early diagnosis. The purpose of this study was to develop techniques for identifying cancer biomarkers in the serum of patients with pancreatic cancer. Methods Serum samples from five individuals with pancreatic cancer and five individuals without cancer were compared. High-amount serum p...